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RPE proteins resolved by <t>2D</t> <t>gel</t> electrophoresis. Representative flamingo-stained gels (125 μ g) from the (a) aging and the (b) AMD comparison. Spots indicated showed altered content with each process. A linear range of pI 5 to 8 is indicated at the top and molecular weights of the standard are marked on the left.
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RPE proteins resolved by <t>2D</t> <t>gel</t> electrophoresis. Representative flamingo-stained gels (125 μ g) from the (a) aging and the (b) AMD comparison. Spots indicated showed altered content with each process. A linear range of pI 5 to 8 is indicated at the top and molecular weights of the standard are marked on the left.
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RPE proteins resolved by <t>2D</t> <t>gel</t> electrophoresis. Representative flamingo-stained gels (125 μ g) from the (a) aging and the (b) AMD comparison. Spots indicated showed altered content with each process. A linear range of pI 5 to 8 is indicated at the top and molecular weights of the standard are marked on the left.
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RPE proteins resolved by <t>2D</t> <t>gel</t> electrophoresis. Representative flamingo-stained gels (125 μ g) from the (a) aging and the (b) AMD comparison. Spots indicated showed altered content with each process. A linear range of pI 5 to 8 is indicated at the top and molecular weights of the standard are marked on the left.
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RPE proteins resolved by <t>2D</t> <t>gel</t> electrophoresis. Representative flamingo-stained gels (125 μ g) from the (a) aging and the (b) AMD comparison. Spots indicated showed altered content with each process. A linear range of pI 5 to 8 is indicated at the top and molecular weights of the standard are marked on the left.
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RPE proteins resolved by 2D gel electrophoresis. Representative flamingo-stained gels (125 μ g) from the (a) aging and the (b) AMD comparison. Spots indicated showed altered content with each process. A linear range of pI 5 to 8 is indicated at the top and molecular weights of the standard are marked on the left.

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Dissecting Regulators of Aging and Age-Related Macular Degeneration in the Retinal Pigment Epithelium

doi: 10.1155/2022/6009787

Figure Lengend Snippet: RPE proteins resolved by 2D gel electrophoresis. Representative flamingo-stained gels (125 μ g) from the (a) aging and the (b) AMD comparison. Spots indicated showed altered content with each process. A linear range of pI 5 to 8 is indicated at the top and molecular weights of the standard are marked on the left.

Article Snippet: Spot alignment and density quantification were performed using a 2D gel analysis software (PDQuest 7.1.1; Bio-Rad).

Techniques: Two-Dimensional Gel Electrophoresis, Electrophoresis, Staining, Comparison